Do Not Stop! Run! is a casual, endless runner. The player directs a large-headed, naked red toddler or baby to run and glide across a rapidly changing two-tone, green checkboard landscape over hills and around and above trees and boulders. Their goal is to direct the figure as far as possible to achieve the longest distance for the run. They also receive a reward of coins based on the maximum score distance and maximum speed of the avatar.
When the player directs the figure to start running, a speedometer appears on the screen. The game also displays the current score in meters. The player can make the figure weave around trees, boulders and other objects. Once they make it run at a fast enough speed off a hill, they can direct it to fly and glide down the sloping landscape. If the figure hits an obstacle by accident, the run ends. They can continue with a recovery by watching an ad video.
Do Not Stop! Run! allows the player to randomly upgrade the appearance of the runner with coins or ad video views via a blue button marked with a shopping cart icon on the main screen. They can change the figure's color and give it headwear. The latter options include a crown, a miner's hard hat, a policeman's cap, a cowboy hat, a top hat, a sombrero and a two-horned helmet.